Welcome to the Quantitative Microbial Physiology and Ecology (QMPE) conference 2025!Research advances in microbial physiology have yielded new knowledge on the coordination of cell growth, DNA replication, nucleoid segregation, and cell division. Meanwhile, recent discoveries in microbial ecology have revealed how microbes respond and adapt to changing environments. Bridging these , this conference offers a unique and intimate platform for researchers in the fields of microbial physiology and ecology, with the aim of sharing insights and exploring potential collaborations. Conference topics include: Spatio-temporal coordination of bacterial DNA replication, mass growth and cell division Gene regulatory networks and physical processes underlying microbial physiology Microbial response and adaptation in changing environments Ecology and evolution of microbial communities